Determination of fumonisins in maize by High Performance Liquid Chromatography with fluorescence and ultraviolet detection of o-phthaldialdehyde, naphthalene-2,3-dicarboxaldehyde and dansyl chloride derivatives

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dc.contributor.advisorGreen, Ivan R
dc.contributor.advisorShephard, Gordon
dc.contributor.advisorvan der Westhuizen, Liana
dc.contributor.authorNdube, Ncediwe
dc.contributor.otherDept. of Chemistry
dc.contributor.otherFaculty of Science
dc.date.accessioned2013-07-31T06:57:40Z
dc.date.accessioned2024-05-09T10:49:58Z
dc.date.available2011/11/07 13:59
dc.date.available2011/11/07
dc.date.available2013-07-31T06:57:40Z
dc.date.available2024-05-09T10:49:58Z
dc.date.issued2011
dc.descriptionMasters of Scienceen_US
dc.description.abstractFumonisins, carcinogenic mycotoxins produced by various Fusarium species, occur naturally in maize and maize-based food products. They are hazards for animal and human health as they cause cancer in rodents and have been associated with oesophageal cancer and neural tube defects in humans. The most abundant naturally occurring fumonisins analogues in maize are fumonisin B1, B2 and B3 (FB1, FB2 and FB3). For analytical determination, they mostly require suitable extraction, clean-up and pre or post-column derivatization together with reversed-phase HPLC separation. o- Phthaldialdehyde (OPA) had been adopted as the most widely used derivatization reagent for fumonisins as they lack useful chromophores or fluorophores. Alternative derivatization reagents, naphthalene-2,3- dicarboxaldehyde (NDA) and dansyl chloride (DnS-Cl), were investigated in this study.en_US
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/14447
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectFumonisinsen_US
dc.subjectFluorescence detectoren_US
dc.subjectUltraviolet detectoren_US
dc.subjectOphthaldialdehydeen_US
dc.subjectNaphthalene-2,3-dicarboxaldehydeen_US
dc.subjectDansyl chlorideen_US
dc.subjectHighperformance liquid chromatographyen_US
dc.subjectStrong anion extractionen_US
dc.subjectImmunoaffinityen_US
dc.subjectDiode array detectoren_US
dc.titleDetermination of fumonisins in maize by High Performance Liquid Chromatography with fluorescence and ultraviolet detection of o-phthaldialdehyde, naphthalene-2,3-dicarboxaldehyde and dansyl chloride derivativesen_US
dc.typeThesisen_US

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