Investigation of the role of the ubiquitin-like DWNN domain in targeting Retinoblastoma Binding Protein 6 to nuclear speckles
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Date
2018
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
University of the Western Cape
Abstract
Magister Scientiae - MSc (Biotechnology)
Description
Retinoblastoma Binding Protein 6 (RBBP6) is a 200 KDa protein shown to play a role in 3'-
polyadenylation of mRNA transcripts, as well as to function as an E3 ligase catalysing
ubiquitination of cancer-associated proteins. RBBP6 has been previously reported to localise to
nuclear speckles, which are thought to play a role in mRNA splicing, presumably as a result of
its RS domain, which is known to target mRNA splicing factors to nuclear speckles. However
recent studies in our laboratory have shown that isoform 3 of RBBP6, consisting mainly of the
DWNN domain, also localises to speckles in resting cells, but more strongly in cells subjected to
various stresses, suggesting that the DWNN domain may be the speckle-targeting domain.
Keywords
RBBP6, DWNN domain, Protein localisation, Site-directed mutagenesis, Nuclear speckles