Comparative in vitro transportation of pentamidine across the blood-brain barrier using polycaprolactone nanoparticles and phosphatidylcholine liposomes

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dc.contributor.authorKippie, Y
dc.date.accessioned2022-12-05T10:46:36Z
dc.date.available2022-12-05T10:46:36Z
dc.date.issued2019
dc.description.abstractNanoparticles (NPs) have gained importance in addressing drug delivery challenges across biological barriers. Here, we reformulated pentamidine, a drug used to treat Human African Trypanosomiasis (HAT) in polymer based nanoparticles and liposomes and compared their capability to enhance pentamidine penetration across blood brain barrier (BBB). Size, polydispersity index, zeta potential, morphology, pentamidine loading and drug release profiles were determined by various methods. Cytotoxicity was tested against the immortalized mouse brain endothelioma cells over 96 h. Moreover, cells monolayer integrity and transportation ability were examined for 24 h. Pentamidine-loaded polycaprolactone (PCL) nanoparticles had a mean size of 267.58, PDI of 0.25 and zeta potential of –28.1 mV and pentamidine-loaded liposomes had a mean size of 119.61 nm, PDI of 0.25 and zeta potential 11.78. Pentamidine loading was 0.16 µg/mg (w/w) and 0.17 µg/mg (w/w) in PCL NPs and liposomes respectively. PCL nanoparticles and liposomes released 12.13% and 22.21% of pentamidine respectively after 24 h. Liposomes transported 87% of the dose, PCL NPs 66% of the dose and free pentamidine penetration was 63% of the dose. These results suggest that liposomes are comparatively promising nanocarriers for transportation of pentamidine across BBB.en_US
dc.identifier.otherhttps://doi.org/10.1080/21691401.2019.1596923
dc.identifier.urihttp://hdl.handle.net/10566/8212
dc.language.isoenen_US
dc.publisherUWCen_US
dc.subjectpentamidineen_US
dc.subjectPCL nanoparticlesen_US
dc.subjectliposomesen_US
dc.subjectblood brain barrieren_US
dc.subjecttransendothelial electrical resistanceen_US
dc.titleComparative in vitro transportation of pentamidine across the blood-brain barrier using polycaprolactone nanoparticles and phosphatidylcholine liposomesen_US
dc.typeArticleen_US

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