Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro

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dc.contributor.authorHenkel, R.R
dc.contributor.authorMoichela, F.T
dc.contributor.authorAdefolaju, G.A
dc.date.accessioned2021-05-31T08:53:49Z
dc.date.available2021-05-31T08:53:49Z
dc.date.issued2021
dc.description.abstractThe effects of aqueous leaf extract of Moringa oleifera (MO) on human sperm functions and integrity was studied in vitro. Semen was obtained by masturbation after 3–5 days' abstinence from 34 healthy donors in Western Cape, South Africa. Liquefied semen was washed in human tubular fluid supplemented with 1% bovine serum albumin (HTF-BSA;1:5) with 10 min centrifugation at 300 g. Sperm suspensions were subsequently incubated with MO extract (0.625, 6.25, 62.5 and 625 µg/ml) for 1 hr, where HTF-BSA served as control. Sperm motility, vitality, DNA fragmentation, reactive oxygen species production, mitochondrial membrane potential, capacitation and acrosome reaction were assessed. Sperm motility, vitality, mitochondrial membrane potential and capacitation remained unchanged (p >.05). A dose-dependent decrease in sperm reactive oxygen species production (p <.0001), DNA fragmentation (p <.0001) and acrosome reaction (p <.001) was observed. An increase in the percentage of non-capacitated sperm (p <.01) was noted at 625 µg/ml.en_US
dc.identifier.citationHenkel, R.R. et al. (2021). Aqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitro. Andrologia, 53(1),e13903en_US
dc.identifier.issn0303-4569
dc.identifier.uri10.1111/and.13903
dc.identifier.urihttp://hdl.handle.net/10566/6224
dc.language.isoenen_US
dc.publisherBlackwell Publishing Ltden_US
dc.subjectAntioxidantsen_US
dc.subjectInfertilityen_US
dc.subjectMoringa oleiferaen_US
dc.subjectReactive oxygen speciesen_US
dc.subjectSpermatozoaen_US
dc.titleAqueous leaf extract of Moringa oleifera reduced intracellular ROS production, DNA fragmentation and acrosome reaction in Human spermatozoa in vitroen_US
dc.typeArticleen_US

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