Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT

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dc.contributor.authorFielding, Burtram C.
dc.contributor.authorGunalan, Vithiagaran
dc.contributor.authorTan, Timothy H.P.
dc.contributor.authorChou, Chih-Fong
dc.contributor.authorShen, Shuo
dc.contributor.authorKhan, Sehaam
dc.contributor.authorLim, Seng Gee
dc.contributor.authorHong, Wanjin
dc.contributor.authorTan, Yee-Joo
dc.date.accessioned2013-11-28T11:02:46Z
dc.date.available2013-11-28T11:02:46Z
dc.date.issued2006
dc.description.abstractSevere acute respiratory syndrome coronavirus (SARS-CoV) 7a is an accessory protein with no known homologues. In this study, we report the interaction of a SARS-CoV 7a and small glutamine-rich tetratricopeptide repeat-containing protein (SGT). SARS-CoV 7a and human SGT interaction was identified using a two-hybrid system screen and confirmed with interaction screens in cell culture and cellular co-localization studies. The SGT domain of interaction was mapped by deletion mutant analysis and results indicated that tetratricopeptide repeat 2 (aa 125-158) was essential for interaction. We also showed that 7a interacted with SARS-CoV structural proteins M (membrane) and E (envelope), which have been shown to be essential for virus-like particle formation. Taken together, our results coupled with data from studies of the interaction between SGT and HIV-1 vpu indicated that SGT could be involved in the life-cycle, possibly assembly of SARS-CoV.en_US
dc.description.accreditationISIen_US
dc.identifier.citationFielding, B.C., et al. (2006). Severe acute respiratory syndrome coronavirus protein 7a interacts with hSGT. Biochemical and Biophysical Research Communications, 343: 1201-1208en_US
dc.identifier.issn0006-291X
dc.identifier.urihttp://hdl.handle.net/10566/882
dc.identifier.urihttp://dx.doi.org/10.1016/j.bbrc.2006.03.091
dc.language.isoenen_US
dc.privacy.showsubmitterfalse
dc.publisherElsevieren_US
dc.rightsThis is the post-print version of the article published available online at http://dx.doi.org/10.1016/j.bbrc.2006.03.091
dc.status.ispeerreviewedtrue
dc.subjectSevere acute respiratory syndrome coronavirusen_US
dc.subjectProtein–protein interactionen_US
dc.subjectAccessory proteinen_US
dc.subjectSmall glutamine-rich tetratricopeptide repeat-containing proteinen_US
dc.titleSevere acute respiratory syndrome coronavirus protein 7a interacts with hSGTen_US
dc.typeArticleen_US

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