Real-time assessment of interspecies candida biofilm formation

dc.contributor.authorAbrantes, Pedro MDS
dc.contributor.authorBehardien, Kauthar
dc.contributor.authorAfrica, Charlene WJ
dc.date.accessioned2023-03-16T07:39:26Z
dc.date.available2023-03-16T07:39:26Z
dc.date.issued2022-12-30
dc.description.abstractBackground: Candida infections are responsible for increased morbidity and mortality in immunocompromised patients, with Candida having the ability to form highly structured biofilms that protect them from the external environment and the action of antifungals. Few studies have reported on real-time interactions between Candida species. Objective: This study aimed to investigate the interspecies biofilm changes of oral Candida species using the xCELLigence system and the CV staining technique. Methods: This study demonstrated the mixed biofilm interactions of Candida species using an impedance-based biofilm monitoring system and crystal violet staining. Results: When using the xCELLigence system, the maximum cell index increased in most mixed biofilms, except for the C. glabrata/C. parapsilosis and C. albicans combinations. Bulk biofilm formation measured by CV staining was the highest in C. albicans and C. tropicalis combinations and the lowest in the C. glabrata/C. parapsilosis combination. Pseudohyphae were observed in C. albicans and C. glabrata in combination with C. tropicalis or C. parapsilosis. Conclusion: This study is the first to report on the real-time interactions of Candida species using the xCELLigence system and suggests that the presence of specific species influences the biofilm formation of commonly isolated Candida species.en_US
dc.description.sponsorshipThe National Research Foundation of South Africa partially supported this study financially. Any opinion, findings, and conclusions or recommendations expressed in this material are those of the authors, and therefore, the NRF does not accept any liability in regards thereto.en_US
dc.identifier.citationPedro MDS Abrantes, Kauthar Behardien, Charlene WJ Africa. “Real-time assessment of interspecies Candida biofilm formation”. Open Microbiol J. 16: e187428582211230, 2022. doi: 10.2174/18742858-v16-e221128-2022-26.en_US
dc.identifier.otherdoi: 10.2174/18742858-v16-e221128-2022-26
dc.identifier.urihttp://hdl.handle.net/10566/8596
dc.language.isoenen_US
dc.publisherBentham Openen_US
dc.subjectCandidaen_US
dc.subjectBiofilmsen_US
dc.subjectCell adhesionen_US
dc.subjectCV stainingen_US
dc.subjectxCELLigence systemen_US
dc.subjectSpecies interactionsen_US
dc.titleReal-time assessment of interspecies candida biofilm formationen_US
dc.typeArticleen_US

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