Three Novel Bacteria Associated with Two Centric Diatom Species from the Mediterranean Sea, Thalassiosira rotula and Skeletonema marinoi

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dc.contributor.authorvan Zyl, LJ
dc.contributor.authorDi Costanzo, F
dc.contributor.authorDi Dato, V
dc.date.accessioned2022-03-10T15:59:20Z
dc.date.available2022-03-10T15:59:20Z
dc.date.issued2021
dc.description.abstractDiatoms are a successful group of microalgae at the base of the marine food web. For hundreds of millions of years, they have shared common habitats with bacteria, which favored the onset of interactions at different levels, potentially driving the synthesis of biologically active molecules. To unveil their presence, we sequenced the genomes of bacteria associated with the centric diatom Thalassiosira rotula from the Gulf of Naples. Annotation of the metagenome and its analysis allowed the reconstruction of three bacterial genomes that belong to currently undescribed species. Their investigation showed the existence of novel gene clusters coding for new polyketide molecules, antibiotics, antibiotic-resistance genes and an ectoine production pathway. Real-time PCR was used to investigate the association of these bacteria with three different diatom clones and revealed their preference for T. rotula FE80 and Skeletonema marinoi FE7, but not S. marinoi FE60 from the North Adriatic Sea. Additionally, we demonstrate that although all three bacteria could be detected in the culture supernatant (free-living), their number is up to 45 times higher in the cell associated fraction, suggesting a close association between these bacteria and their host. We demonstrate that axenic cultures of T. rotula are unable to grow in medium with low salinity (<28 ppt NaCl) whereas xenic cultures can tolerate up to 40 ppt NaCl with concomitant ectoine production, likely by the associated bacteria.en_US
dc.identifier.citationDi Costanzo, Federica & Dato, Valeria & Zyl, Leonardo & Cutignano, Adele & Esposito, Francesco & Tuffin, Marla & Romano, Giovanna. (2021). Three Novel Bacteria Associated with Two Centric Diatom Species from the Mediterranean Sea, Thalassiosira rotula and Skeletonema marinoi. International Journal of Molecular Sciences. 22. 13199. 10.3390/ijms222413199.en_US
dc.identifier.issn1422-0067
dc.identifier.uri/10.3390/ijms222413199
dc.identifier.urihttp://hdl.handle.net/10566/7313
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectdiatomsen_US
dc.subjectmarine bacteriaen_US
dc.subjectmetagenomeen_US
dc.subjectbioinformaticsen_US
dc.titleThree Novel Bacteria Associated with Two Centric Diatom Species from the Mediterranean Sea, Thalassiosira rotula and Skeletonema marinoien_US
dc.typeArticleen_US

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