Aptameric recognition-modulated electroactivity of poly(4-styrenesolfonic acid)-doped polyaniline films for single-shot detection of tetrodotoxin

Simple item page
dc.contributor.advisorFomo, Gertrude
dc.contributor.advisorWaryo, Tesfaye T.
dc.contributor.advisorSunday, Christopher Edozie
dc.contributor.advisorBaleg, Abd A.
dc.contributor.advisorBaker, Priscilla
dc.contributor.advisorIwuoha, Emmanuel I.
dc.date.accessioned2017-02-02T13:47:47Z
dc.date.available2017-02-02T13:47:47Z
dc.date.issued2015
dc.description.abstractThe work being reported is the first electrochemical sensor for tetrodotoxin (TTX). It was developed on a glassy carbon electrodes (C) that was modified with poly(4-styrenesolfonic acid)-doped polyaniline film (PANI/PSSA). An amine-end functionalized TTX-binding aptamer, 5′-NH2-AAAAATTTCACACGGGTGCCTCGGCTGTCC-3′ (NH2-Apt), was grafted via covalent glutaraldehyde (glu) cross-linking. The resulting aptasensor (C//PANI+/PSSA-glu-NH2-Apt) was interrogated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in sodium acetate buffer (NaOAc, pH 4.8) before and after 30 min incubation in standard TTX solutions. Both CV and EIS results confirmed that the binding of the analyte to the immobilized aptamer modulated the electrochemical properties of the sensor: particularly the charge transfer resistance (Rct) of the PANI+/PSSA film, which served as a signal reporter. Based on the Rct calibration curve of the TTX aptasensor, the values of the dynamic linear range (DLR), sensitivity and limit of detection (LOD) of the sensor were determined to be 0.23–1.07 ng·mL−1 TTX, 134.88 ± 11.42 Ω·ng·mL−1 and 0.199 ng·mL−1 , respectively. Further studies are being planned to improve the DLR as well as to evaluate selectivity and matrix effects in real samples.en_US
dc.description.accreditationISIen_US
dc.identifier.citationFomo, G. et al. (2015). Aptameric recognition-modulated electroactivity of poly(4-styrenesolfonic acid)-doped polyaniline films for single-shot detection of tetrodotoxin. Sensors, 15: 22547-22560en_US
dc.identifier.issn1424-8220
dc.identifier.urihttp://hdl.handle.net/10566/2518
dc.identifier.urihttp://dx.doi.org/10.3390/s150922547
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAll articles published by MDPI are made immediately available worldwide under an open access license
dc.subjectTetrodotoxin sensoren_US
dc.subjectAptameren_US
dc.subjectPolyanilineen_US
dc.subjectPuffer fishen_US
dc.titleAptameric recognition-modulated electroactivity of poly(4-styrenesolfonic acid)-doped polyaniline films for single-shot detection of tetrodotoxinen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Fomo_Aptameric Recognition_2015.pdf
Size:
1.5 MB
Format:
Adobe Portable Document Format
Description:
Download

Collections

Research Articles (Chemistry)