Diversity of dsDNA viruses in a South African hot spring assessed by metagenomics and microscopy
| dc.contributor.author | Zablocki, Olivier | |
| dc.contributor.author | van Zyl, Leonardo Joaquim | |
| dc.contributor.author | Kirby, Bronwyn | |
| dc.contributor.author | Trindade, Marla | |
| dc.date.accessioned | 2017-12-13T10:09:15Z | |
| dc.date.available | 2017-12-13T10:09:15Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | The current view of virus diversity in terrestrial hot springs is limited to a few sampling sites. To expand our current understanding of hot spring viral community diversity, this study aimed to investigate the first African hot spring (Brandvlei hot spring; 60 C, pH 5.7) by means of electron microscopy and sequencing of the virus fraction. Microscopy analysis revealed a mixture of regular- and ‘jumbo’-sized tailed morphotypes (Caudovirales), lemon-shaped virions (Fuselloviridae-like; salterprovirus-like) and pleiomorphic virus-like particles. Metavirome analysis corroborated the presence of His1-like viruses and has expanded the current clade of salterproviruses using a polymerase B gene phylogeny. The most represented viral contig was to a cyanophage genome fragment, which may underline basic ecosystem functioning provided by these viruses. Furthermore, a putative Gemmata-related phage was assembled with high coverage, a previously undocumented phage-host association. This study demonstrated that a moderately thermophilic spring environment contained a highly novel pool of viruses and should encourage future characterization of a wider temperature range of hot springs throughout the world. | en_US |
| dc.identifier.citation | Zablocki, O. et al. (2017). Diversity of dsDNA viruses in a South African hot spring assessed by metagenomics and microscopy. Viruses, 9: 384 | en_US |
| dc.identifier.issn | 1999-4915 | |
| dc.identifier.uri | http://dx.doi.org/10.3390/v9110348 | |
| dc.identifier.uri | http://hdl.handle.net/10566/3328 | |
| dc.language.iso | en | en_US |
| dc.privacy.showsubmitter | FALSE | |
| dc.publisher | MDPI | en_US |
| dc.rights | © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |
| dc.status.ispeerreviewed | TRUE | |
| dc.subject | Hot spring | en_US |
| dc.subject | Metavirome | en_US |
| dc.subject | Fuselloviridae | en_US |
| dc.subject | Jumbo phage | en_US |
| dc.subject | Archaeal viruses | en_US |
| dc.subject | Freshwater Cyanophages | en_US |
| dc.subject | Gemmata | en_US |
| dc.title | Diversity of dsDNA viruses in a South African hot spring assessed by metagenomics and microscopy | en_US |
| dc.type | Article | en_US |