The arabidopsis wall associated kinase-like 10 gene encodes a functional guanylyl cyclase and is coexpressed with pathogen defense related genes
Loading...
Date
2010
Journal Title
Journal ISSN
Volume Title
Publisher
Public Library of Science
Abstract
Second messengers have a key role in linking environmental stimuli to physiological responses. One such messenger, guanosine 3′,5′-cyclic monophosphate (cGMP), has long been known to be an essential signaling molecule in many different physiological processes in higher plants, including biotic stress responses. To date, however, the guanylyl cyclase (GC) enzymes that catalyze the formation of cGMP from GTP have largely remained elusive in higher plants. We have identified an Arabidopsis receptor type wall associated kinase–like molecule (AtWAKL10) as a candidate GC and provide experimental evidence to show that the intracellular domain of AtWAKL10431–700 can generate cGMP in vitro. Further, we also demonstrate that the molecule has kinase activity indicating that AtWAKL10 is a twin-domain catalytic protein. A co-expression and stimulus-specific expression analysis revealed that AtWAKL10 is consistently co-expressed with well characterized pathogen defense related genes and along with these genes is induced early and sharply in response to a range of pathogens and their elicitors.
Description
Keywords
Biotechnology, Gene expression, Microarrays, Signaling molecules, Arabidopsis thaliana
Citation
Meier, S. et al. (2010). The arabidopsis wall associated kinase-like 10 gene encodes a functional guanylyl cyclase and is coexpressed with pathogen defense related genes. PLoS ONE, 5(1). https://doi.org/10.1371/journal.pone.0008904