Colistin, Carbapenem and Cephalosporin-resistant Klebsiella pneumoniae reported from Misrata, Libya

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dc.contributor.authorShallouf, Mohamed
dc.contributor.authorAbrantes, Pedro Miguel dos Santos
dc.contributor.authorFielding, Burtram Clinton
dc.contributor.authorAfrica, Charlene Wilma Joyce
dc.date.accessioned2019-03-06T06:15:58Z
dc.date.available2019-03-06T06:15:58Z
dc.date.issued2017
dc.description.abstractBackground: National surveillance of antimicrobial resistance has become a mandatory approach to control the spread of antimicrobial resistance and for the establishment of antibiotic treatment guidelines. In this study, clinical isolates of K. pneumoniae were phenotypically investigated for the presences of Colistin and beta-lactams resistance. Methods: Clinical samples were obtained from hospitalized (n=140) and non-hospitalized patients (n=60) in Misrata, Libya. Identification of the isolated species was achieved using the VITEK 2 compact system. Screening for Carbapenem and Cephalosporin-resistance was performed using the disk diffusion method with Carbapenem (10µg) and Cephalosporin (30µg) disks and Minimum Inhibitory Concentration (MIC) determined by VITEK 2. Colistin resistance was determined using both Sensititre Gram-negative Xtra plate format (GNX2F) and VITEK 2. Carbapenemase activity was detected using the RAPIDEC CARBA NP, Modified Hodge test, Carbapenem inactivation method, MAST Combi Carba plus kit (D73C) and Meropenem combined disk test. ESBL and AmpC production was confirmed using Sensititre ESBL confirmatory plates (ESB1F), modified double disk synergy test MDDST, MAST ESBL detection kit D67C, AmpC & ESBL detection kit D68C along with AmpC detection kit D69C. Results and conclusion: Of the 200 clinical isolates, 85 (42.5%) were K. pneumoniae of which 54 (63.52%) demonstrated resistance to at least one of the Carbapenems, 16 (18.82%) were ESBL or AmpC producers and 2 (2.35%) were Carbapenem and Colistin resistant. 13 (21.25%) isolates were susceptible to all antibiotics tested except Ampicillin and Augmentin.en_US
dc.identifier.citationShallouf MA, Abrantes PMDS, Fielding BC, Africa CWJ. (2017). Candida species carriage in diabetic patients in Misrata, Libya. Southern African Journal of Infectious Diseases. 32(4):ID8387en_US
dc.identifier.issn2312-0053
dc.identifier.urihttp://hdl.handle.net/10566/4349
dc.language.isoenen_US
dc.publisherMedpharm Publicationsen_US
dc.relation.ispartofseriesFederation of Infectious Diseases Societies of Southern Africa 7th Congress;ID8387
dc.subjectDrug resistanceen_US
dc.subjectKlebsiella pneumoniaeen_US
dc.subjectLibyaen_US
dc.titleColistin, Carbapenem and Cephalosporin-resistant Klebsiella pneumoniae reported from Misrata, Libyaen_US
dc.typePresentationen_US

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