Evaluation of protein purification techniques and effects of storage duration on lc-ms/ms analysis of archived ffpe human crc tissues

dc.contributor.authorRossouw, Sophia C.
dc.contributor.authorBendou, Hocine
dc.contributor.authorBlignaut, Renette J.
dc.date.accessioned2021-10-14T12:02:37Z
dc.date.available2021-10-14T12:02:37Z
dc.date.issued2021
dc.description.abstractTo elucidate cancer pathogenesis and its mechanisms at the molecular level, the collecting and characterization of large individual patient tissue cohorts are required. Since most pathology institutes routinely preserve biopsy tissues by standardized methods of formalin fixation and paraffin embedment, these archived FFPE tissues are important collections of pathology material that include patient metadata, such as medical history and treatments. FFPE blocks can be stored under ambient conditions for decades, while retaining cellular morphology, due to modifications induced by formalin. However, the effect of long-term storage, at resource-limited institutions in developing countries, on extractable protein quantity/quality has not yet been investigated. In addition, the optimal sample preparation techniques required for accurate and reproducible results from label-free LC-MS/MS analysis across block ages remains unclear. This study investigated protein extraction efficiency of 1, 5, and 10-year old human colorectal carcinoma resection tissue and assessed three different gel-free protein purification methods for label-free LC-MS/MS analysis. A sample size of n 17 patients per experimental group (with experiment power 0.7 and α 0.05, resulting in 70% confidence level) was selected.en_US
dc.identifier.citationRossouw, S. C. et al. (2021). Evaluation of protein purification techniques and effects of storage duration on lc-ms/ms analysis of archived ffpe human crc tissues. Pathology and Oncology Research, 27. https://doi.org/10.3389/pore.2021.622855en_US
dc.identifier.issn1532-2807
dc.identifier.uri10.3389/pore.2021.622855
dc.identifier.urihttp://hdl.handle.net/10566/6913
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.subjectProteinen_US
dc.subjectCanceren_US
dc.subjectPatient tissueen_US
dc.subjectParaffinen_US
dc.titleEvaluation of protein purification techniques and effects of storage duration on lc-ms/ms analysis of archived ffpe human crc tissuesen_US
dc.typeArticleen_US

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