Oranzie, MarlonJanuary, Jaymi LSanga, Nelia A.Leve, Zandile DMini, SixolileCupido, CandiceDouman, Samantha FIwuoha, Emmanuel I.2026-01-282026-01-282025Oranzie, M., January, J.L., Sanga, N.A., Leve, Z.D., Mini, S., Cupido, C., Douman, S.F. and Iwuoha, E.I., 2025. Aptamer‐Driven Biosensor Technology for the Quantitative Analysis of C‐Reactive Protein. ChemElectroChem, 12(7), p.e202400667.https://doi.org/10.1002/celc.202400667https://hdl.handle.net/10566/21874C-reactive protein (CRP) is a widely recognized biomarker for early myocardial infarction (MI) detection, released into the bloodstream during heart inflammation. Traditional assays for CRP detection, like ELISA and immunoradiometric assays, are costly, time-consuming, and require large sample volumes. Aptasensors are becoming increasingly popular for MI diagnosis due to their affordability, simplicity, and potential for point-of-care use. In this study, an electrochemical aptasensor incorporating mercaptosuccinic acid-capped nickel selenide quantum dots (MSA-NiSe2 QD) were developed for CRP detection. The amine-modified aptamer was immobilized on the MSA-NiSe2 QD using EDC/NHS coupling chemistry. Chronocoulometric measurements showed high selectivity towards CRP in phosphate buffer, with a linear range of 10–110 pg/mL and a detection limit of 2.80 pg/mL. Cross-reactivity experiments confirmed the aptasensor's high selectivity for CRP. Testing in human serum samples demonstrated recovery rates of 94–100.5 %, indicating its suitability for clinical diagnostics. Validation studies with a commercial CRP ELISA kit showed the aptasensor's superior sensitivity in both physiological buffer and human serum.enC-reactive protein (CRP)Chronocoulometry (CC)Electrochemical aptasensorMyocardial infarction (MI)Quantum dot (QD)Article