Mulaudzi-Masuku, TakalaniMutepe, Rendani DaphneyMukhoro, Ofhani Christopher2023-06-072023-06-072015Mulaudzi-Masuku, T. et al. (2015). Identification and characterization of a heat-inducible Hsp70 gene from Sorghum bicolor which confers tolerance to thermal stress. Cell Stress and Chaperones, 20, 793–804. https://doi.org/10.1007/s12192-015-0591-21466-126810.1007/s12192-015-0591-2http://hdl.handle.net/10566/9051This study describes the first detailed molecular characterization of the heat shock protein 70 (Hsp70) gene from Sorghum bicolor, MN1618 designated as SbHsp70-1. The full-length cDNA of SbHsp70-1 consists of 2524 bp with a 1950 bp open reading frame, which encodes a protein of 649 amino acids. SbHsp70-1 is a cytoplasmic protein with high homology to other plant Hsp70s, especially grain crops. Recombinant SbHsp70-1 was able to bind and hydrolyse ATP in a dose-dependent manner, suggesting that SbHsp70-1 functions as an ATPase.enBiotechnologySorghum bicolorAgricultureMedicinal plantsArticle