Ali, Ali Elnaeim ElbasheirHusselmann, Lizex HollenbachTabb, David L.2023-03-282023-03-282023Ali, A. E. E. et al. (2023). Comparative proteomics analysis between maize and sorghum uncovers important proteins and metabolic pathways mediating drought tolerance. Life, 13(1), 170. https://doi.org/10.3390/life130101702075-1729https://doi.org/10.3390/life13010170http://hdl.handle.net/10566/8679Drought severely affects crop yield and yield stability. Maize and sorghum are major crops in Africa and globally, and both are negatively impacted by drought. However, sorghum has a better ability to withstand drought than maize. Consequently, this study identifies differences between maize and sorghum grown in water deficit conditions, and identifies proteins associated with drought tolerance in these plant species. Leaf relative water content and proline content were measured, and label-free proteomics analysis was carried out to identify differences in protein expression in the two species in response to water deficit. Water deficit enhanced the proline accumulation in sorghum roots to a higher degree than in maize, and this higher accumulation was associated with enhanced water retention in sorghum. Proteomic analyses identified proteins with differing expression patterns between the two species, revealing key metabolic pathways that explain the better drought tolerance of sorghum than maize.enProteomic analysisBiotechnologyDrought stressSorghum bicolorSouth AfricaArticle