Serpionov, Genrikh V.Molokanova, Ludmila GNikolskaya, Daria VDrozhzhin, Nikita AVinogradov, Iliya IRossouw, ArnouxAndreev, Evgeny VOrelovich, Oleg LPetrik, Leslie FNechaev, Alexander N2026-05-162026-05-162026Serpionov, G.V., Molokanova, L.G., Nikolskaya, D.V., Drozhzhin, N.A., Vinogradov, I.I., Rossouw, A., Andreev, E.V., Orelovich, O.L., Petrik, L.F., Nechaev, A.N. and Apel, P.Y., 2026. Effects of morphological and physicochemical surface properties of track-etched membranes made from polyethylene terephthalate, polycarbonate and polyethylene naphthalate on protein adsorption. Colloids and Surfaces A: Physicochemical and Engineering Aspects, p.140300.https://doi.org/10.1016/j.colsurfa.2026.140300https://hdl.handle.net/10566/22472This study presents a comparative analysis of bovine serum albumin (BSA), hemoglobin, and lysozyme adsorption on track-etched membranes (TMs) based on polyethylene terephthalate (PET), polyethylene naphthalate (PEN), and polycarbonate (PC) with similar structural parameters. The specific surface area was 1.7, 1.9, and 1.3 m²·g⁻¹ for PEN, PET, and PC TMs, respectively. PEN and PET TMs were rougher (Sq ≈ 6.2–9.4 nm) and hydrophilic (contact angle ≈55°), whereas PC TMs were smoother (Sq ' 2.8 nm) and moderately hydrophobic (contact angle '80°). All membranes were negatively charged (zeta potential: –14.9 to –23.6 mV). No measurable BSA adsorption was observed using the spectrophotometric method. Hemoglobin adsorption followed PEN ' PET ' PC, with capacities of 7.9, 6.4, and 3.4 mg·g⁻¹ , respectively, all fitted by the Langmuir isotherm. Lysozyme adsorption was substantially higher on PEN (9.9 mg·g⁻¹) and PET (9.3 mg·g⁻¹) than on PC (2.3 mg·g⁻¹). Adsorption on PEN was best described by the Jovanović model, on PET by the Temkin isotherm, and on PC by the Langmuir model, indicating different adsorption behaviours. The novelty of this work lies in the multimethodological and multimodel approach that identifies surface roughness, protein molecular size and electrostatic interactions as key factors governing protein adsorption on TMs. Protein adsorption on PEN-based TMs is reported for the first time. This study shows that protein adsorption on TMs is relevant not only to fouling in filtration but also to biosensing and cell culture, providing a basis for rational selection of TM materials for biomedical and separation applications.enHemoglobinLysozymePolycarbonatePolyethylene naphthalatePolyethylene terephthalateArticle