Iwuoha, EmmanuelBaker, P.G.L.Somerse, Vernon SydwillDept. of ChemistryFaculty of Science2014-02-092024-05-132010/01/262010/01/262014-02-092024-05-132007https://hdl.handle.net/10566/14778Philosophiae Doctor - PhDThis study firstly reports the development, characterisation, and application of thick-film acetylcholinesterase (AChE) biosensors based on a gold electrode modified with a mercaptobenzothiazole (MBT) self-assembled monolayer and either poly(omethoxyaniline) (POMA) or poly(2,5-dimethoxyaniline) (PDMA) in the presence of polystyrene(4-sulphonic acid) (PSSA). The Au/MBT/POMA-PSSA/AChE and Au/MBT/PDMA-PSSA/AChE biosensors were then applied to successfully detect standard organophosphorous and carbamate pesticides in a 0.1 M phosphate buffer, 0.1 M KCl (pH 7.2) solution. Secondly, it reports the construction of the Au/MBT/PANI/AChE/PVAc thick-film biosensor for the determination of certain organophosphate and carbamate pesticide solutions in selected aqueous organic solvent solutions.enMercaptobenzothiazolePolyanilineAcetylcholinesteraseSelf-Assembled MonolayerOrganophosphatesCarbamatesPesticidesOrganic Phase Amperometric BiosensorNanomolar Detection LimitVoltammetryThesisUniversity of the Western Cape