Iranpour, Farhad GolshanNateghian, ZohreHenkel, Ralf2023-06-072023-06-072019Iranpour, F. G. et al. (2019). Effects of temperature and storage time on the motility, viability, DNA integrity and apoptosis of processed human spermatozoa. Andrologia, 52 (2). https://doi.org/10.1111/and.134851439-0272https://doi.org/10.1111/and.13485http://hdl.handle.net/10566/9049The aim of this study was to evaluate motility, viability, DNA integrity and apoptosis of spermatozoa when washed semen samples were kept for up to 12 days at 4–6°C and 25°C. In this experimental study, 26 normozoospermic semen samples were washed twice in Modified Ham's F10 and resuspended in IVF fertilisation medium. Half of the specimens were stored at 4–6°C, and the other half was kept at 25°C for 12 days. The proportions of viable, motile, spermatozoa with double-stranded DNA and apoptotic spermatozoa were examined during storage time. Apoptosis was measured using annexin V-PI staining followed by flow cytometry. Results showed that sperm motility and viability decreased during 12 days of sample storage (p < .001). There was no significant difference between the two temperatures in terms of motility and viability for up to 2 days (p < .05).enBioscienceDNA fragmentationSperm motilityHuman geneticsMedical SciencesArticle