van Zyl, LJDell'Anno, FTrindade, M2023-03-292023-03-292023Dell’Anno, F., van Zyl, L.J., Trindade, M., Buschi, E., Cannavacciuolo, A., Pepi, M., Sansone, C., Brunet, C., Ianora, A., de Pascale, D. and Golyshin, P.N., 2023. Microbiome enrichment from contaminated marine sediments unveils novel bacterial strains for petroleum hydrocarbon and heavy metal bioremediation. Environmental Pollution, 317, p.120772.02697491http://hdl.handle.net/10566/8700Petroleum hydrocarbons and heavy metals are some of the most widespread contaminants affecting marine ecosystems, urgently needing effective and sustainable remediation solutions. Microbial-based bioremediation is gaining increasing interest as an effective, economically and environmentally sustainable strategy. Here, we hypothesized that the heavily polluted coastal area facing the Sarno River mouth, which discharges >3 tons of polycyclic aromatic hydrocarbons (PAHs) and ~15 tons of heavy metals (HMs) into the sea annually, hosts unique microbiomes including marine bacteria useful for PAHs and HMs bioremediation. We thus enriched the microbiome of marine sediments, contextually selecting for HM-resistant bacteria. The enriched mixed bacterial culture was subjected to whole-DNA sequencing, metagenome-assembled-genomes (MAGs) annotation, and further sub-culturing to obtain the major bacterial species as pure strains.enpolycyclic aromatic hydrocarbonsheavy metalsbioremediationmarine biotechnologynext-generation sequencingArticle