Keyster, MarshallWu, Ruomou2018-08-152024-05-092021-08-312024-05-092018https://hdl.handle.net/10566/13464Magister Scientiae - MSc (Biotechnology)The yearly increase of global population will result in a greater demand for crop production, but with the climates changes and a lack of available agricultural land it will become increasingly more difficult to provide sufficient crops to feed everyone adequately. Application of the PGPE has proven over the past researches to be able enhance growth of plants via various growth promoting mechanisms. To identify suitable growth promoting bacteria candidate, E. plantagineum plant was used to isolate endophytes from the root after surface sterilization. The isolates bacteria were used to inoculate Brassica napus L seeds. The effects of isolate's ability to promote growth were evaluated based on the certain growth parameters after 42 days in the green house. Isolate CP5 produced highest results in all growth parameter. Isolates CP5 was selected as potential candidate as significant improvement was shown by this isolate. This isolate was tested for the ability to produce ACC deaminase, solubilize phosphate, synthesize IAA and siderophore production. Furthermore isolate CP5 growth promotion abilities was tested on Brassica napus L under antimony stress.enBrasscia napus L, plant growth promoting endophytic bacteria, endophytes, Echium plantagineum, ACC deaminase, Indole-3-acetic acid, phosphate solubilization, siderophore production, antimonyUniversity of the Western Cape