Green, Ivan RShephard, Gordonvan der Westhuizen, LianaNdube, NcediweDept. of ChemistryFaculty of Science2013-07-312024-05-092011/11/072011/11/072013-07-312024-05-092011https://hdl.handle.net/10566/14447Masters of ScienceFumonisins, carcinogenic mycotoxins produced by various Fusarium species, occur naturally in maize and maize-based food products. They are hazards for animal and human health as they cause cancer in rodents and have been associated with oesophageal cancer and neural tube defects in humans. The most abundant naturally occurring fumonisins analogues in maize are fumonisin B1, B2 and B3 (FB1, FB2 and FB3). For analytical determination, they mostly require suitable extraction, clean-up and pre or post-column derivatization together with reversed-phase HPLC separation. o- Phthaldialdehyde (OPA) had been adopted as the most widely used derivatization reagent for fumonisins as they lack useful chromophores or fluorophores. Alternative derivatization reagents, naphthalene-2,3- dicarboxaldehyde (NDA) and dansyl chloride (DnS-Cl), were investigated in this study.enFumonisinsFluorescence detectorUltraviolet detectorOphthaldialdehydeNaphthalene-2,3-dicarboxaldehydeDansyl chlorideHighperformance liquid chromatographyStrong anion extractionImmunoaffinityDiode array detectorThesisUniversity of the Western Cape