Henkel, RalfArafa, Mohamed MostafaAgarwal, Ashok2021-01-072021-01-072020Henkel, R. et al. (2020). Seminal oxidation–reduction potential levels are not influenced by the presence of leucocytospermia. Andrologia, 52(7),e13609 11439-027110.1111/and.13609http://hdl.handle.net/10566/5585Oxidative stress (OS) is characterised by an excessive amount of reactive oxygen species (ROS) which negatively affect sperm functions. In this study, the influence of leucocytes on seminal oxidation–reduction potential (ORP) and sperm DNA fragmentation (SDF) was investigated in 1,068 men. Seminal leucocyte concentration did not correlate with SDF, unadjusted ORP, ORP normalised for sperm concentration (sORP), ORP normalised for total motile sperm concentration (motORP) or total motile sperm count (TMSC-ORP). Although receiver operator characteristic (ROC) curve analyses show that leucocytospermia does not predict high sORP values (>1.34 mV/106 spermatozoa/ml), the motORP (AUC: 0.666) and TMSC-ORP (AUC: 0.683) predict the rate of leucocytospermia significantly (p =.0195 and p =.0085 respectively). Moreover, SDF can significantly predict leucocytospermia (AUC: 0.679; p =.011) and vice versa (AUC: 0.657, p =.0298). Our data confirm the association between OS and SDF. In conclusion, motORP and TMSC-ORP may be better predictive factors of leucocytospermia, probably because sperm motility, included in motORP and TMSC-ORP calculation, is the first seminal parameter to be affected by OS. Although all these parameters are indicative of OS, ORP values, SDF and leucocytospermia should be considered independently for the evaluation of redox seminal status, as they probe distinct seminal features.enLeucocytospermiaMale infertilityOxidation–reduction potentialOxidative stressSperm DNA damageArticle