Mazloum, Alivan Schalkwyk, AntoinetteChernyshev, Roman2023-04-172023-04-172023Mazloum, A. et al. (2023). A guide to molecular characterization of genotype ii African swine fever virus: Essential and alternative genome markers. Microorganisms, 11(3), 642. https://doi.org/10.3390/microorganisms110306422076-2607https://doi.org/10.3390/microorganisms11030642http://hdl.handle.net/10566/8823African swine fever is a contagious viral disease that has been spreading through Europe and Asia since its initial report from Georgia in 2007. Due to the large genome size of the causative agent, the African swine fever virus (ASFV), the molecular epidemiology, and virus evolution are analyzed by employing different markers. Most of these markers originate from single nucleotide polymorphisms or disparities in the copy number of tandem repeat sequences observed during the comparisons of full genome sequences produced from ASFVs isolated during different outbreaks. Therefore, consistent complete genome sequencing and comparative analysis of the sequence data are important to add innovative genomic markers that contribute to the delineation of ASFV phylogeny and molecular epidemiology during active circulation in the field. In this study, the molecular markers currently employed to assess the genotype II ASFVs circulating in Europe and Asia have been outlined.enAfricaSwine fever virusPublic healthPhylogenyBiotechnologyArticle