Kemmegne-Mbouguen, Justin ClaudeNgameni, EmmanuelBaker, PriscillaWaryo, Tesfaye T.Kgarebe, BoitumeloIwuoha, Emmanuel I.2017-12-012017-12-012014Kemmegne-Mbouguen, J.C. et al. (2014). Carcinoembryonic antigen immunosensor developed with organoclay nanogold composite film. International Journal of Electrochemical Science, 9: 478 - 4921452-3981http://hdl.handle.net/10566/3292Organoclay nanogold composite were prepared using gold nanoparticles and the natural Cameroonian clay grafted with amino organosilane. The functionnalization of clay provided abundant amino group to assemble gold nanoparticles. A label-free electrochemical immunosensor for the sensitive determination of carcinoembryonic antigen (CEA) was fabricated by immobilizing anti-CEA onto organoclay nanogold composite film modified electrode by the cross-linking method using glutaraldehyde. In addition, the preparation procedure of the immunosensor was investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimal conditions, the resulting immunosensor displayed a high sensitivity for the detection of CEA, and responded to the CEA concentration in two ranges from 0.05 to 5.0 ng/mL (R = 0.991) and from 5.0 to 120.0 ng/mL (R = 0.998) with a detection limit of 0.01 ng/mL.enInternational Journal of Electrochemical Science (ISSN 1452-3981) is international peer-reviewed and open access journalOrganoclayNanogold compositeModified electrodeImmunosenorCarcinoembryonic antigenArticle