Choi, Yook-WahDutta, SujitFielding, Burtram C.Tan, Yee-Joo2014-01-052014-01-052010Choi, Y., et al. (2010). Expression, purification and preliminary crystallographic analysis of recombinant human DEAD-box polypeptide 5. Acta Crystallographica Section F, 66(2): 192-1941744-3091http://hdl.handle.net/10566/912The DEAD-box RNA helicase DDX5 is involved in many aspects of RNA processing and has been implicated in a number of cellular processes involving alteration of RNA secondary structure. The N-terminal region of DDX5, which contains the conserved domain 1 of the DEAD-box helicases, has been cloned and expressed in Escherichia coli and purified. Here, the crystallization and preliminary diffraction analysis of this region is reported. X-ray diffraction data were processed to a resolution of 2.7 A ° . The crystals belonged to space group I222, with unit-cell parameters a = 66.18, b = 73.80, c = 104.00 A ° , = = = 90 .enCopyright International Union of Crystallography. This file may be freely used for educational purposes, as long as it is not altered in any way. Acknowledgement of the authors and the source is required.ExpressionPurificationCrystallizationArticle