Mbamalu, OluchiAntunes, E.Silosini, N.Samsodien, HalimaSyce, James2016-05-122016-05-122016Mbamalu, O.N. et al. (2016). HPLC determination of selected flavonoid glycosides and their corresponding aglycones in Sutherlandia frutescens materials. Medicinal & Aromatic Plants, 5(3): 1-102167-0412http://hdl.handle.net/10566/2183http://dx.doi.org/10.4172/2167-0412.1000246Sutherlandia frutescens is a popular South African plant commercially available in a range of formulations. However, reference standards for quality and stability assessment are lacking. This work reports the development and validation of a reversed phase HPLC method for the analysis of flavonoid glycosides and their corresponding aglycones in S. frutescens products. Five materials containing either leaf powder (LP) or spray-dried aqueous extract (SDAE) of S. frutescens were analysed for flavonoid content. A primary objective was to isolate non-commercially available flavonoid glycoside compounds (sutherlandins) for use as reference standards. Sutherlandins A, B, C and D were successfully isolated, and used, with other flavonoid compounds for HPLC assay development. The developed HPLC method was linear in the range of 0.2 to 60 µg/ml for quercitrin; 0.2 to 120 µg/ml for quercetin and kaempferol; 0.2 to 200 µg/ml for rutin and kaempferol-3-O-rutinoside; 4 to 180 µg/ml for sutherlandins A and D; and 4 to 200 µg/ml for sutherlandins B and C. Percentage content of sutherlandins A, B, C and D, quercetin and kaempferol in different plant materials were significantly different (P<0.001). The developed HPLC method is simple, precise and robust; and can be employed for the simultaneous determination of flavonoid glycosides and aglycones for quality control of S. frutescens products.enThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Sutherlandia frutescensLegumeFlavonoidHerbal medicineQuality controlValidationHigh-performance liquid chromatography (HPLC)Article