Ajayi, Rachel F.Tshobeni, Ziyanda Zamaswazi2022-05-172024-05-092024-05-092022https://hdl.handle.net/10566/14444>Magister Scientiae - MScTuberculosis (TB) carries on being a major global health issue and it is responsible for 1.5 million disease-related mortality in 2020, according to the World Health organisation (WHO). One of the major causes of death among TB patients is the development of drug-resistant TB due to the inappropriate dosage of the treatment drug, non-adherence to the prescribed regimen or using low quality medication. A fast and reliable detection method (such as biosensing) for real-time determination of a patient’s TB drug metabolism profile is therefore very important for ensuring appropriate prescription. In this study, an electrochemical nanobiosensor for the determination of the TB drug, isoniazid also known as isonicotinic acid hydrazide (INH) was developed. It consists of a cytochrome P450-2E1 (CYP2E1) isoenzyme covalently attached on a gold electrode (screen printed or disk) that was functionalized with thioglycolic acid-capped gold nanoparticle (TGA-AuNP), which was green-synthesized with banana peel extract (BPE) as the reducing agent.enGreen synthesisGold nanoparticleThioglycolic acidTuberculosisDrugsUniversity of the Western Cape