Duga, Meschac Musampa2026-01-152026-01-152025https://hdl.handle.net/10566/21724The rising occurrence of tropical neglected diseases and the fast spread of viral infections emphasizes the need for the development of rapid, accurate, selective, sensitive, and cheap analytical devices such as genosensors (biosensors) for early diagnosis in the point of cares (PoC) as per the world health organization (WHO) recommendations. Toward the development of the first iono-amperometric nanoenzymatically amplified sandwich RNA-genosensors, electrochemical sensor platforms for urea based on a seldom employed electrochemical transduction technique, traditionally known as ion transfer voltammetry at the interface between two immiscible electrolyte solutions (ITIES), were constructed and characterized as follows. An NH4+ ion sensor was assembled according to literature by placing a hydrophobic electrolyte phase (O-phase) containing dibenzo-18-crown-6 (DB18C6, 50mM), an NH4+-selective ionophore (selectophore), and ethyl-violet tetraphenylborate (EthVTPB, 10 mM), in a teflon tube, the end of which was sealed with semi-permeable cellulose membrane (CM).enAmmonium Ion Transfer Amperometric SensorUrea Agnps-ITIES BiosensorUrease/Urea Signal AmplificationPolarizable Liquid-Liquid Interface GenosensorDnananoag- EnzymeThesis