Henkel, Ralf2023-01-112023-01-112019Hassanen, E, Elqusi, K, Zaki, H, Henkel, R, Agarwal, A. TUNEL assay: Establishing a sperm DNA fragmentation cut-off value for Egyptian infertile men. Andrologia. 2019; 51:e13375. https://doi.org/10.1111/and.133750303-4569http://hdl.handle.net/10566/8255Male factor infertility is responsible for half of all infertility cases. Conventional semen analysis is inadequate to evaluate male fertility. Sperm DNA fragmentation (SDF) test can be done by: direct methods such as Terminal deoxynucleotidyl transferase dUTP Nick-End Labeling (TUNEL) and Comet assay, or indirect like Sperm Chromatin Structure Assay (SCSA) and Sperm Chromatin Dispersion (SCD). TUNEL assay measures both single- and double-strand breaks and is technically less demanding, while SCSA tests for the susceptibility for nuclear DNA denaturation and samples should be sent to the reference lab. Studies showed that a single cut-off value does not fit all.en-USmale infertilitySDF cut‐off valuesperm DNA fragmentationTunel assayArticle