Ramoba, Lesetja VNzondomyo, Wakopo JSerala, KaraboMacharia, Lucy WBiswas, SupratimPrince, SharonMalan, Frederick PAlexander, Orbett TManicum, Amanda-Lee E2026-05-212026-05-212025Ramoba, L.V., Nzondomyo, W.J., Serala, K., Macharia, L.W., Biswas, S., Prince, S., Malan, F.P., Alexander, O.T. and Manicum, A.L.E., 2025. Derivatives of pyrazole-based compounds as prospective cancer agents. ACS omega, 10(12), pp.12671-12678.https://doi.org/10.1021/acsomega.5c00320?urlappend=%3Fref%3DPDF&jav=VoR&rel=cite-ashttps://hdl.handle.net/10566/22783Five pyrazole-based compounds, 3,5-dimethyl-1H-pyrazole, L1; 3,5-diphenyl-1H-pyrazole, L2; 3-(trifluoromethyl)-5-phenyl-1H-pyrazole, L3; 3- (trifluoromethyl)-5-methyl-1H-pyrazole, L4; and 3,5-ditert-butyl-1H-pyrazole, L5 were synthesized from a typical condensation reaction of β-diketone derivatives with hydrazine hydrate reagent and characterized using various spectroscopic techniques such as FT-IR, UV−vis, 1H and 13C NMR, and LC−MS spectroscopy. L1 was further analyzed by single-crystal X-ray diffraction, and the N1−N1′ bond distance was found to be 1.361(3) Å and correlated well with other pyrazole-based compounds. The short-term cytotoxicity of 10 μM pyrazole compounds (L1−L5) was evaluated against pancreatic (CFPAC-1 and PANC-1), breast (MDA-MB-231 and MCF-7), and cervical (CaSki and HeLa) cancer cell lines using the MTT cell viability assay. Cisplatin and gemcitabine were included as positive control drugs followed by the determination of the half-maximal effective concentrations of prospective compounds. L2 and L3, respectively, displayed moderate cytotoxicity against CFPAC-1 (61.7 ± 4.9 μM) and MCF-7 (81.48 ± 0.89 μM) cell lines.enDiphenylCytotoxicityTrifluoromethylSpectroscopicCisplatinArticle