Ajayi, Rachel F.January, Jaymi Leigh2022-05-172024-05-092024-05-092022https://hdl.handle.net/10566/14466>Magister Scientiae - MScLapatinib is a highly prescribed drug used in combination with other drugs to treat breast cancer. Due to drug toxicity-related side effects experienced by some patients, there is need to determine appropriate dosage for patients. A novel nanobiosensor for the determination of the biotransformation of a cancer drug (LAPA) was constructed with a co-polymer, poly(anilino-co-4-aminobenzoic acid) {poly(ANI-co-4-ABA)} coupled to ground coffee extract-based, green-synthesized indium nanoparticles (InNPs) to which cytochrome P450-3A4 (CYP3A4) enzyme was additionally attached onto a glassy carbon electrode. Polyaniline (PANI), 4-ABA, indium nanoparticles (InNPs), poly(ANI-co-4-ABA) and poly(ANI-co-4-ABA)/InNPs were synthesized and used as sensor materials. The x-ray diffraction (XRD) analysis indicated crystallinity (average crystallite size of 27.31 nm) and gave a sharp peak at 21.40 (211) that is indexed to indium metal.enAnti-cancer drugBreast cancerpulse voltammetry Green synthesisIndium nanoparticlesBiosensorUniversity of the Western Cape