Berry, MichaelManasse, Taryn-LeeTan, Yee-JooFielding, Burtram C.2013-10-062013-10-062012Berry, M., et al. (2012). Characterisation of human coronavirus-NL63 nucleocapsid protein. African Journal of Biotechnology, 11(75): 13962-139681684–5315http://hdl.handle.net/10566/735Coronavirus N is a multifunctional protein that plays an essential role in enhancing the efficiency of virus transcription and assembly. This manuscript reports the analysis of HCoV-NL63 N protein by comparing the amino acid sequences of coronavirus N-homologues. A ~50 kDa protein was expressed in both a mammalian cell and bacterial cell system that is similar in size to the predicted ~42.6 kDa HCoV-NL63 N protein. PSORTII identified two putative nuclear localisations signals and PONDR identified one disordered region in HCoV-NL63 N. The reported protein analysis serves as a prelude to laboratory analysis to understand the processing of HCoV-NL63 N.en© 2012 Berry, et al; licensee Academic Journals. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.HCoV-NL63Nucleocapsid proteinProtein analysisNuclear localisation signalsProtein ordered/disordered regionsArticle