Goh, Phuay-YeeChoi, Yook-WahShen, ShuoTan, Yee-JooFielding, Burtram C.Tan, Timothy H.P.O, Eng-EongLim, Seng GeeHong, Wanjin2014-01-052014-01-052004Goh, P., et al. (2004). Cellular Characterization of SARS Coronavirus Nucleocapsid. EXCLI Journal, 3: 91-1041611-2156http://hdl.handle.net/10566/913The Severe and Acute Respiratory Syndrome coronavirus (SARS CoV) is a newly-emerged virus that caused an outbreak of atypical pneumonia in the winter of 2002-2003. Polyclonal antibodies raised against the nucleocapsid (N) of the SARS CoV showed the localization of N to the cytoplasm and the nucleolus in virus-infected and N-expressing Vero E6 cells. Like other coronavirus N proteins, the SARS N is probably a phosphoprotein. N protein expressed in mammalian cells is apparently able to “spread” to neighboring cells. For N to spread to neighboring cells, it must be exported out of the expressing cells. This is shown by the immunoprecipitation of N from the culture medium of a stable cell line expressing myc-N. Deletion studies showed that the 27 kD C-terminal domain of N (C1/2) is the minimal region of N that can spread to other cells. The nucleolar localization and spreading of N are artefacts of fixation, reminiscent of other protein-transduction domain (PTD)-containing proteinsen© 2004 Goh et al; licensee Leibniz Research Centre for Working Environment and Human Factors. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.SARSCoronavirusNucleocapsidNucleolusTransductionFixationArticle