Puckree-Padua, C.AGabrielson, P.WManeveldt, G.W2021-05-242021-05-242021Puckree-Padua, C.A. et al. (2021). DNA sequencing reveals three new species of Chamberlainium (Corallinales, Rhodophyta) from South Africa, all formerly passing under Spongites yendoi. Botanica Marina, 64(1),19-400006-805510.1515/bot-2020-0074http://hdl.handle.net/10566/6186Three new non-geniculate coralline algal species from South Africa are described that were passing under the misapplied name, Spongites yendoi. Based on plastid encoded DNA sequences from psbA and rbcL markers, these species belong in the subfamily Chamberlainoideae. The DNA sequences, supported by the morpho-anatomical character of tetrasporangial conceptacle roof development, placed all three species in the genus Chamberlainium and not Pneophyllum, the only other genus in Chamberlainoideae. In addition to the diagnostic DNA sequences, Chamberlainium capense sp. nov., C. glebosum sp. nov. and Chamberlainium occidentale sp. nov. may be distinguished by a combination of habit, habitat, geographic distribution, and several morpho-anatomical features. Biogeographically all three species are found in the Benguela Marine Province of South Africa, with C. occidentale being the most widespread. Chamberlainium glebosum also has a wide, but disjunct distribution and C.enBiogeographyChamberlainoideaeCryptic diversityMorpho-anatomyNon-geniculate coralline algaeArticle