Kasu, MohaiminRistow, Peter GustavBurrows, Adria Michelle2024-11-142024-11-142024Kasu, M., Ristow, P.G., Burrows, A.M., Kuplik, Z., Gibbons, M.J. and D'Amato, M.E., 2024. Novel buffer for long-term preservation of DNA in biological material at room temperature. BioTechniques, 76(8), pp.357-370.https://doi.org/10.33232/001c.118782https://hdl.handle.net/10566/19684The collection and preservation of biological material before DNA analysis is critical for inter alia biomedical research, medical diagnostics, forensics and biodiversity conservation. In this study, we evaluate an in-house formulated buffer called the Forensic DNA Laboratory-buffer (FDL-buffer) for preservation of biological material for long term at room temperature. Human saliva stored in the buffer for 8 years, human blood stored for 3 years and delicate animal tissues from the jellyfish Pelagia noctiluca comb jelly Beroe sp., stored for 4 and 6 years respectively consistently produced high-quality DNA. FDL-buffer exhibited compatibility with standard organic, salting out and spin-column extraction methods, making it versatile and applicable to a wide range of applications, including automationenbloodDNA extractionDNA preservationFDL-bufferjellyfishArticle