Baker, Priscilla G.L.Mangombo, Zelo2023-06-122024-05-092023-06-122024-05-092010https://hdl.handle.net/10566/14596>Magister Scientiae - MScThe aim of this project was to develop a biosensor on a modified boron-doped diamond (BDD) platform in order to determine L-phenylalanine (L-Phe) levels in the aqueous media and real samples. A BDD electrode modified with polyaniline (PANI) film doped with polyvinyl sulphonate (PVS), containing in-situ deposited tyrosinase (Tyr) enzyme was studied with special emphasis on the detection of L-phenylalanine (L-Phe). The PANI-PVS film was electrochemically coated on the BDD surface by cyclic voltammetry (CV) at 50 mV s-1 (versus Ag/AgCI). An increase in current density with increasing number of cycles was an indication of the polymer growth. The morphologies of the composite films were characterized by scanning electron microscopy (SEM).enPolyanilineElectrochemical enzyme biosensorChemistryCyclic voltammetryLipropoteinUniversity of the Western Cape