Martin-Sancho, LauraLewinski, Mary K.Shaw, Megan L.2022-07-192022-07-192021Martin-Sancho, L. et al. (2021). Functional landscape of SARS-CoV-2 cellular restriction. Molecular Cell, 81(12), 2656 - 2668. 10.1016/j.molcel.2021.04.0081097-416410.1016/j.molcel.2021.04.008http://hdl.handle.net/10566/7616A deficient interferon (IFN) response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has been implicated as a determinant of severe coronavirus disease 2019 (COVID-19). To identify the molecular effectors that govern IFN control of SARS-CoV-2 infection, we conducted a large-scale gain-of-function analysis that evaluated the impact of human IFN-stimulated genes (ISGs) on viral replication. A limited subset of ISGs were found to control viral infection, including endosomal factors inhibiting viral entry, RNA binding proteins suppressing viral RNA synthesis, and a highly enriched cluster of endoplasmic reticulum (ER)/Golgi-resident ISGs inhibiting viral assembly/egress.enCovid-19Innate immunityProtein bindingTumorBioscienceArticle