Browsing by Author "Roux, Saartjie"
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Item Investigation of bioactive compounds in Crassocephalum rubens leaf and in vitro anticancer activity of its biosynthesized gold nanoparticles(Elsevier, 2020) Roux, Saartjie; Adewale, Olusola Bolaji; Potts-Johnson, Sotonye S.The development of cancer therapies has become difficult due to high metastasis, and lack of tissue selectivity, which in most cases affects normal cells. Demand for anticancer therapy is therefore increasing on daily basis. Gold nanoparticles (AuNPs) have many applications in biomedical field. Biological synthesis of AuNPs using aqueous extract of Crassocephalum rubens (AECR) was designed to investigate the in vitro anticancer potential. The synthesized AuNPs were characterized by UV–vis spectroscopy, high-resolution transmission electron microscopy, and Fourier transform infrared spectroscopy. The characterization results showed the formation of green AuNPs of wavelength 538 nm, and mostly spherical AuNPs with 20 5 nm size. Significant anticancer activity of the AECRAuNPs on MCF-7 and Caco-2 cells was noted at higher concentrations (125 and 250mg/mL) during 24 and at all concentrations tested during 48 h. It can therefore be concluded that AECR leaves can mediate stable AuNPs with anticancer properties.Item One-pot synthesis, characterisation and biological activities of gold nanoparticles prepared using aqueous seed extract of Garcinia kola(2023-02) Sibuyi, Nicole R.S.; Fadaka, Adewale O.; Anadozie, Scholastica O.; Adewale, Olusola B.; Isitua, Chinwe C.; Davids, Hajierah; Roux, SaartjieRecently, biogenic synthesis of gold nanoparticles (AuNPs) has become a focus area in cancer research owing to the eco-friendliness and cost effectiveness of the synthetic method. In this study, aqueous extract of Garcinia kola seed (AEGKs) was used for the bio-reduction of Au3+ to Au0. The synthesised AEGKs-AuNPs was characterised by ultraviolet-visible (UV-Vis) spectroscopy, dynamic light scattering (DLS), high-resolution transmission electron microscopy (HRTEM) and Fourier transform-infrared (FT-IR) spectroscopy. The in vitro antioxidant activity of the AEGKs and AEGKs-AuNPs was evaluated using 2,2-diphenyl-1-picrylhydrazyl radical scavenging ability and ferric reducing antioxidant power assays. The AEGKs-AuNPs showed an absorption maximum at 512 nm, and the HRTEM images revealed mostly, spherical-shaped AuNPs in the size range of 2–17 nm. The FT-IR spectroscopy revealed that polyphenolic compounds and proteins were predominant, and responsible for the reduction and capping of the AuNPs. The AEGKs-AuNPs showed concentration dependent antioxidant activities, while dose dependent in vitro anti-cancer activity of the AEGKs-AuNPs was demonstrated against lungs, prostrate, human cervical and human colon cancer cells, using the 3-(4,5-dimethylthiazol-2-yl)− 2,5-diphenyltetrazolium bromide tetrazolium reduction (MTT) assay. The antioxidant and anti-cancer activities of the AEGKs-AuNPs could be attributed to the presence of phytochemicals and physicochemical properties of the AuNPs.