Browsing by Author "Masiga, Daniel K."
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Item Human African trypanosomiasis research gets booost: unravelling the tsetse Genome(PLOS, 2014) Aksoy, Serap; Attardo, Geoffrey; Berriman, Matthew; Christoffels, Alan; Lehane, Mike; Masiga, Daniel K.; Toure', YeyaHuman African trypanosomiasis (HAT), also known as sleeping sickness, is a neglected disease that impacts 70 million people distributed over 1.55 million km2 in sub-Saharan Africa. Trypanosoma brucei gambiense accounts for almost 90% of the infections in central and western Africa, the remaining infections being from T. b. rhodesiense in eastern Africa. Furthermore, the animal diseases caused by related parasites inflict major economic losses to countries already strained. The parasites are transmitted to the mammalian hosts through the bite of an infected tsetse fly.Item International Glossina Genome Initiative 2004-2014: a driver for post-genomic era research on the African continent(PLOS, 2014) Christoffels, Alan; Masiga, Daniel K.; Berriman, Matthew; Lehane, Mike; Toure', Yeya; Aksoy, SerapHuman African trypanosomiasis (HAT), also known as sleeping sickness, is a neglected disease that impacts 70 million people distributed over 1.55 million km2 in sub- Saharan Africa and includes at least 50% of the population of theDemocratic Republic of the Congo [1]. Trypanosoma brucei gambiense accounts for more than 98% of the infections in central and West Africa, the remaining infections being from Trypanosoma brucei rhodesiense in East Africa [2]. The parasites are transmitted to the hosts through the bite of an infected tsetse fly. Disease control is challenging as there are no vaccines, and effective, easily delivered drugs are still lacking. Treatment invariably involves lengthy hospitalization, with both medical and socioeconomic consequences.Item Molecular detection of tick-borne pathogen diversities in ticks from livestock and reptiles along the shores and adjacent islands of lake Victoria and lake Baringo, Kenya(Frontiers Media, 2017) Omondi, David; Masiga, Daniel K.; Fielding, Burtram C.; Kariuki, Edward; Ajamma, Yvonne UkamakaTick-borne pathogens (TBPs) are responsible for some of the most serious emerging infectious diseases facing sub-Saharan Africa (SSA) and the rest of the world today (1, 2). In Kenya, TBPs (including viral diseases—arboviruses) like Crimean Congo hemorrhagic fever (CCHF), Dugbe, Kupe, and Hazara, as well as hemoparasites that cause babesiosis, theileriosis, and rickettsiosis, are major impediments to livestock productivity and public health.Item Odorant and gustatory receptors in the tsetse fly Glossina morsitans morsitans(PLOS, 2014) Obiero, George F.; Nyanjom, Steven R. G.; Mireji, Paul O.; Christoffels, Alan; Robertson, Hugh M.; Masiga, Daniel K.Tsetse flies use olfactory and gustatory responses, through odorant and gustatory receptors (ORs and GRs), to interact with their environment. Glossina morsitans morsitans genome ORs and GRs were annotated using homologs of these genes in Drosophila melanogaster and an ab initio approach based on OR and GR specific motifs in G. m. morsitans gene models coupled to gene ontology (GO). Phylogenetic relationships among the ORs or GRs and the homologs were determined using Maximum Likelihood estimates. Relative expression levels among the G. m. morsitans ORs or GRs were established using RNA-seq data derived from adult female fly. Overall, 46 and 14 putative G. m. morsitans ORs and GRs respectively were recovered. These were reduced by 12 and 59 ORs and GRs respectively compared to D. melanogaster. Six of the ORs were homologous to a single D. melanogaster OR (DmOr67d) associated with mating deterrence in females. Sweet taste GRs, present in all the other Diptera, were not recovered in G. m. morsitans. The GRs associated with detection of CO2 were conserved in G. m. morsitans relative to D. melanogaster. RNA-sequence data analysis revealed expression of GmmOR15 locus represented over 90% of expression profiles for the ORs. The G. m. morsitans ORs or GRs were phylogenetically closer to those in D. melanogaster than to other insects assessed. We found the chemoreceptor repertoire in G. m. morsitans smaller than other Diptera, and we postulate that this may be related to the restricted diet of blood-meal for both sexes of tsetse flies. However, the clade of some specific receptors has been expanded, indicative of their potential importance in chemoreception in the tsetse.Item Opportunities in Africa for training in genome science(Academic Journals, 2004) Masiga, Daniel K.; Isokpehi, Raphael D.Genome science is a new type of biology that unites genetics, molecular biology, computational biology and bioinformatics. The availability of the human genome sequence, as well as the genome sequences of several other organisms relevant to health, agriculture and the environment in Africa necessitates the development and delivery of several types and levels of training that will enhance the use of genome data and the associated computational resources. A survey of initiatives that provide opportunities for training in genome science is presented. Current efforts to increase the ability of African scientists to computationally process and analyse genomic and post-genomic data have the potential to produce excellent scientists who perform cutting-edge, hypothesis-based research, and who will accelerate the continent's scientific and technological development.