Browsing by Author "Manogari Chetty"

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    Clinicopathological profile of ameloblastomas among Sudanese patients
    (University of the Western Cape, 2024) Mashail Mahmoud Hamid; Manogari Chetty
    Background: Ameloblastoma is considered the most common benign epithelial odontogenic tumor with a high rate of recurrence if not removed adequately. Knowledge of early events leading to and promoting tumorigenesis in ameloblastoma remains limited, partly because studies directed at identifying molecular factors and events that initiate, and drive tumorigenesis are inconclusive. Exploring the genetic aspects and correlating that to the biological behavior of ameloblastoma and its subtypes in a big sample size may help predict the clinical behavior and prognosis to embark on the most suitable treatment protocol. Moreover, it can objectively assess post-therapeutic response, particularly in recurrent cases of ameloblastoma. Many epidemiological studies on ameloblastoma have been conducted worldwide, however, despite the high frequency and aggressive behavior of this tumor in Sudan, there are no recent published reports regarding the prevalence of ameloblastoma, even though many patients are diagnosed and treated at Khartoum Teaching Dental Hospital (the main OMS referral hospital in Sudan). Most of these patients present with large-sized aggressive tumors with massive bone destruction and soft tissue invasion; cases with recurrence and malignant transformation are often seen there as well. Moreover, several markers and genes involved in the pathogenesis of ameloblastoma have been investigated worldwide; however, no similar studies have been conducted in Sudan, therefore, further investigation and studies in this field are needed.
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    Racemisation of aspartic acid from dentin for chronological age estimation in a South African population using HPLC with UV detection.
    (University of the Western Cape, 2024) Bailie, Keané Catt; Manogari Chetty
    Forensic odontology relies on chronological age assessment to identify unknown people in resource-constrained situations. Due to insufficient DNA profiling and dental data in South Africa, additional procedures must be developed. Aspartic acid racemisation (AAR) in dentin is a promising biochemical age assessment method that may be more accurate and objective. High-Performance Liquid Chromatography (HPLC) with diode-array detection (DAD) was used to test AAR analysis in a South African population. Multiple analytical methods were used to validate dentin samples. Scanning Electron Microscopy (SEM) analysed microstructure, Energy Dispersive X-ray Spectroscopy (EDS) examined elemental composition, Fourier-Transform Infrared spectroscopy (FTIR) validated demineralisation, and X-ray diffraction (XRD) assessed crystallographic characteristics. HPLC analysis used a C8 column with methanol and sodium phosphate buffer gradient elution. Sample validation confirmed the dentin samples' structural and chemical integrity. SEM investigation revealed similar surface features with mean roughness parameters Ra of 11.60864 μm and Rq of 35.63723 μm. EDS showed a homogeneous elemental distribution with oxygen (41.149%), calcium (34.335%), carbon (14.132%), phosphorus (10.955%), magnesium (1.883%), and sodium (0.706%). FTIR spectroscopy revealed the effective isolation of organic and inorganic fractions, whereas XRD validated crystallite sizes in accordance with hydroxyapatite literature values.