Browsing by Author "Burrows, Adria Michelle"

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    A comparative ancestry analysis of Y-chromosome DNA haplogroups using high resolution melting
    (University of the Western Cape, 2018) Burrows, Adria Michelle; D’Amato, Maria Eugenia
    The objective of this study is to deduce paternal ancestry using ancestry informative single nucleotide polymorphisms (SNPs) by means of High Resolution Melting (HRM). This was completed by producing a multiplex system that was designed in a hierarchical manner according to the YSNP tree. This project mainly focused on African ancestry and was used to infer paternal ancestral lineages on the Johannesburg Coloured population. South Africa has a diverse population that has ancestral history from across the globe. The South African Coloured population is the most admixed population as it is derived from at least five different population groups: these being Khoisan, Bantu, Europeans, Indians and Southeast Asians. There have been studies done on the Western Cape/ Cape Town Coloured populations before but this study focused on the Johannesburg Coloured population.
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    Forensic science international: genetics supplement series
    (ScienceDirect, 2019) Burrows, Adria Michelle
    We have designed a buffer for preservation of DNA in biological material at room temperature. In this work we evaluated the integrity of DNA from saliva, stored for 4 years and blood stored for 4 months at room temperature. Parallel tests were conducted with other commercial systems. No sign of degradation was detected in the tested samples.
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    Novel buffer for long-term preservation of DNA in biological material at room temperature
    (Taylor and Francis Ltd., 2024) Kasu, Mohaimin; Ristow, Peter Gustav; Burrows, Adria Michelle
    The collection and preservation of biological material before DNA analysis is critical for inter alia biomedical research, medical diagnostics, forensics and biodiversity conservation. In this study, we evaluate an in-house formulated buffer called the Forensic DNA Laboratory-buffer (FDL-buffer) for preservation of biological material for long term at room temperature. Human saliva stored in the buffer for 8 years, human blood stored for 3 years and delicate animal tissues from the jellyfish Pelagia noctiluca comb jelly Beroe sp., stored for 4 and 6 years respectively consistently produced high-quality DNA. FDL-buffer exhibited compatibility with standard organic, salting out and spin-column extraction methods, making it versatile and applicable to a wide range of applications, including automation